The Thermo Scientific Talos F200C (S)TEM is a 20-200 kV thermionic (scanning) transmission electron microscope uniquely designed for performance and productivity across a wide range of samples and applications, such as 2D and 3D imaging of cells, cell organelles, asbestos, polymers, and soft materials, both at ambient and cryogenic temperatures. It increases the imaging quality of beam-sensitive materials with the optional, motorized, retractable cryo-box and low-dose technique. A side-entry retractable energy dispersive spectroscopy (EDS) detector can also be added to enable chemical analysis. The large C-Twin pole piece gap, which provides high application flexibility, combined with a reproducibly performing electron column, opens up new opportunities for high-resolution 3D characterization, in situ dynamic observations, as well as diffraction applications, with a special emphasis on high-contrast imaging and cryo-TEM. The Talos F200C S/TEM is equipped with the fast 4k × 4k Thermo Scientific Ceta 16M Camera that provides a large field-of-view and fast imaging, with high sensitivity, on a 64-bit platform. In addition, the Thermo Scientific Maps Software enables intuitive image-based navigation over a whole sample and easy correlation of results across imaging platforms.