The Thermo Scientific Glacios Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete cryo-EM solution to a broad range of scientists. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot), as well as the same innovative automation for ease of use as on the Krios G4 Cryo-TEM – all of them bundled into a small footprint that simplifies installation. The designed-in connectivity ensures a robust and contamination-free pathway throughout the entire workflow – from sample preparation and optimization, to image acquisition and data processing. This cryo-TEM can be used in a single particle analysis (SPA) workflow for pre-optimization of sample quality and for high-resolution SPA data acquisition. In addition, the Glacios Cryo-TEM can also be configured with a Direct Electron Detector and/or Volta Phase Plate to be a complete standalone SPA data acquisition solution.